Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Linking genetic variants to bone microstructure: Histological signatures of osteogenesis imperfecta subtypes.

Bone·2026
Same author

Fracture risk assessment by dual-energy absorptiometry in osteogenesis imperfecta: A systematic review of measurement sites and its predictive value.

Bone reports·2026
Same author

Impact of quantified knee positioning on the measurement of minimal joint space width using statistical shape models: A cross-sectional and longitudinal analysis in the IMI-APPROACH.

Osteoarthritis imaging·2026
Same author

Tibial genu varum and primary cam morphology in healthy young adults: A cross-sectional study uncovering the double threat to joint health.

Osteoarthritis and cartilage open·2026
Same author

Harnessing copper-strontium synergy in an injectable composite for multi-pathway bone regeneration.

RSC advances·2026
Same author

The Effects of Radioimmunotherapy and Antibiotics on Biofilm-Associated Implant Infections in a Preclinical Rat Model.

Journal of orthopaedic research : official publication of the Orthopaedic Research Society·2026
Same journal

Deep Learning Based Framework for Detection and Classification of Leukemia Using Microscopic Images.

Microscopy research and technique·2026
Same journal

Externally Controlled In Situ SEM: Multi-Rate Scanning With Signal Regulation and Spatiotemporal Fusion.

Microscopy research and technique·2026
Same journal

In Situ TEM Observation of Phase Transformation Nucleation at the Near-Surface of Synthetic Aragonite.

Microscopy research and technique·2026
Same journal

Morpho-Anatomical and HPTLC Investigations of Lysimachia nummularia L. (Primulaceae) Grown in Switzerland.

Microscopy research and technique·2026
Same journal

Macroscopic, Histological and Ultrastructural Features of the Tongue of the Anatolian Wild Boar (Sus scrofa libycus).

Microscopy research and technique·2026
Same journal

Ultrastructural Insights Into the Reproductive Anatomy and Eggs of Cotton Pink Bollworm, Pectinophora gossypiella Saunders (Lepidoptera: Gelechiidae).

Microscopy research and technique·2026
See all related articles

Related Experiment Video

Updated: Oct 21, 2025

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
07:29

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research

Published on: September 27, 2024

955

Multiscale characterization of pathological bone tissue.

E Deniz Eren1, Wouter H Nijhuis2, Freek van der Weel1

  • 1Laboratory of Physical Chemistry, Department of Chemical Engineering and Chemistry, Eindhoven University of Technology, Eindhoven, The Netherlands.

Microscopy Research and Technique
|September 7, 2021
PubMed
Summary
This summary is machine-generated.

Ultrastructural analysis of bone using combined microscopy reveals critical multiscale details. Three-dimensional imaging offers superior insights into pathological bone tissue compared to two-dimensional methods.

Keywords:
collagenelectron microscopyelectron tomographyfocused ion beamoptical microscopypathological bone tissuesserial slice and viewultrastructure of bone

More Related Videos

Author Spotlight: An Economic and Efficient Method for Quantitative Evaluation of Bone Microarchitecture in a Murine Osteoporosis Model
06:59

Author Spotlight: An Economic and Efficient Method for Quantitative Evaluation of Bone Microarchitecture in a Murine Osteoporosis Model

Published on: September 8, 2023

2.8K
Longitudinal Micro-Computed Tomography Image Analysis for User-Defined Region of Interest in Critical-Sized Bone Defects
08:39

Longitudinal Micro-Computed Tomography Image Analysis for User-Defined Region of Interest in Critical-Sized Bone Defects

Published on: June 24, 2025

285

Related Experiment Videos

Last Updated: Oct 21, 2025

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
07:29

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research

Published on: September 27, 2024

955
Author Spotlight: An Economic and Efficient Method for Quantitative Evaluation of Bone Microarchitecture in a Murine Osteoporosis Model
06:59

Author Spotlight: An Economic and Efficient Method for Quantitative Evaluation of Bone Microarchitecture in a Murine Osteoporosis Model

Published on: September 8, 2023

2.8K
Longitudinal Micro-Computed Tomography Image Analysis for User-Defined Region of Interest in Critical-Sized Bone Defects
08:39

Longitudinal Micro-Computed Tomography Image Analysis for User-Defined Region of Interest in Critical-Sized Bone Defects

Published on: June 24, 2025

285

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Orthopedics

Background:

  • Bone's hierarchical multiscale organization is vital for function.
  • Ultrastructural analysis is key to understanding bone composition, cell communication, and organization.
  • Pathological bone conditions necessitate detailed ultrastructural investigation.

Purpose of the Study:

  • To describe a combined microscopy approach for multiscale bone ultrastructural analysis.
  • To investigate the multiscale organization of pathological bone tissues.
  • To compare the information obtained from 2D and 3D imaging techniques.

Main Methods:

  • Combined optical, scanning electron, and transmission electron microscopy.
  • Top-down approach from macroscale to nanoscale.
  • Quantitative analysis of calcium content, osteocyte spacing, canaliculi, collagen fibrils, and mineral orientation.

Main Results:

  • Detailed ultrastructural and quantitative data were obtained for pathological bone tissues.
  • Two-dimensional (2D) imaging provided comparable information to 3D.
  • Significant differences in observed structural details emerged when analyzing pathological bone in 3D versus 2D.

Conclusions:

  • Combined microscopy effectively characterizes bone ultrastructure across scales.
  • Three-dimensional (3D) imaging provides crucial, distinct insights into pathological bone.
  • Advanced sample preparation and quantitative analysis are vital for electron microscopy of bone.