Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Average Nursing Home Staffing Levels Do Not Reflect Facility-Level Staffing Adequacy.

Journal of the American Medical Directors Association·2026
Same author

A Vascular Invasion-Related Gene Signature Identifies NUP35 as a Driver of Angiogenesis and Poor Prognosis in Pancreatic Ductal Adenocarcinoma.

Biomedicines·2026
Same author

Retinoic acid as a stage-specific modulator of hematopoietic lineage fate from human pluripotent stem cells.

Cell communication and signaling : CCS·2026
Same author

A highly sensitive genetically encoded red cAMP sensor for multiplex imaging in vivo.

Nature communications·2026
Same author

Membrane Protein Folding and Biogenesis: Insights from Single-Molecule Force Spectroscopy.

Chemical reviews·2026
Same author

Exposure to Nanoplastics Disrupts Neurotransmitter Release in Rat Hippocampal Neurons.

Environment & health (Washington, D.C.)·2026

Related Experiment Video

Updated: Oct 21, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.1K

Simultaneous Real-Time Three-Dimensional Localization and FRET Measurement of Two Distinct Particles.

Xingxiang Chen1, Teng Liu2, Xianan Qin2

  • 1Division of Life Science, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong.

Nano Letters
|September 7, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a new 3D microscopy technique to track nanoscale interactions of biological components in real-time. This method precisely localizes and measures interactions, offering new insights into cellular processes like vesicle fusion.

Keywords:
FRETnanometric precisionsingle-particle trackingthree-dimensional localizationvesicle fusion

More Related Videos

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.4K
Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

10.9K

Related Experiment Videos

Last Updated: Oct 21, 2025

A Protocol for Real-time 3D Single Particle Tracking
10:16

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

15.1K
3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles
11:28

3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

Published on: October 1, 2014

10.4K
Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy iPALM

Published on: December 1, 2016

10.9K

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Biological processes rely on component location and interaction.
  • Three-dimensional (3D) spatial information is crucial for understanding these processes.
  • Simultaneous 3D localization and interaction measurement in real-time is a significant technical challenge.

Purpose of the Study:

  • To develop a novel microscopy technique for simultaneous 3D localization and real-time interaction measurement of multiple components.
  • To achieve high spatiotemporal resolution for observing nanoscale dynamics.
  • To apply the technique to study vesicle fusion dynamics.

Main Methods:

  • Developed a new 3D microscopy technique.
  • Utilized fluorescence resonance energy transfer (FRET) for interaction measurement.
  • Achieved tens of nanometers localization accuracy with 100 ms exposure time.
  • Tracked spectrally distinct vesicles containing t-SNAREs or v-SNARE.

Main Results:

  • Successfully localized two spectrally distinct particles in 3D with high accuracy.
  • Simultaneously measured real-time interactions using FRET.
  • Observed nanoscale motion and interactions of single vesicles during fusion events.
  • Provided detailed real-time 3D nanoscale information.

Conclusions:

  • The developed microscope enables simultaneous real-time 3D localization and interaction measurement.
  • The technique offers unprecedented insights into nanoscale dynamics of biological processes like vesicle fusion.
  • This advancement has broad implications for studying molecular mechanisms in cell biology.