Take time to look at the fish: Behavioral response to acute thermal challenge in two Amazonian cichlids

Critical thermal maximum (CT_max ) is often used as an index of upper thermal tolerance in fishes; however, recent studies have shown that some fishes exhibit agitation or avoidance behavior well before the CT_max is reached. In this study, we quantified behavioral changes during CT_max trials in two Amazonian cichlids, Apistogramma agassizii and Mesonauta insignis. The thermal agitation temperature (T_ag ) was recorded as the temperature at which fish left cover and began swimming in an agitated manner, and four behaviors (duration of sheltering, digging, activity, and aquatic surface respiration [ASR]) were compared before and after T_ag . Both A. agassizii and M. insignis exhibited high critical thermal maxima, 40.8°C and 41.3°C, respectively. Agitation temperature was higher in M. insignis (37.3°C) than in A. agassizii (35.4°C), indicating that A. agassizii has a lower temperature threshold at which avoidance behavior is initiated. Activity level increased and shelter use decreased with increased temperatures, and patterns were similar between the two species. Digging behavior increased after T_ag in both species, but was higher in A. agassazii and may reflect its substrate-oriented ecology. ASR (ventilating water at the surface film) was extremely rare before T_ag , but increased in both cichlid species after T_ag and was greater in M. insignis than in A. agassizii. This suggests that fish were experiencing physiological hypoxia at water temperatures approaching CT_max . These results demonstrate that acute thermal challenge can induce a suite of behavioral changes in fishes that may provide additional, ecologically relevant information on thermal tolerance.