Percutaneous penetration and dermal exposure risk assessment of chlorinated paraffins

The widespread occurrence of chlorinated paraffins (CPs) in environmental matrices has resulted in a high frequency exposure to CPs via dermal contact. To quantitatively estimate percutaneous penetration of CPs, Episkin® human skin equivalents (HSE) was applied as an in vitro model to evaluate the mechanism of percutaneous penetration of CPs. The co-exposure of CPs mixtures to HSE showed that about 11.7% and 10.2% of short-chain CPs (SCCPs) and medium-chain CPs (MCCPs) could penetrate the HSE and enter the receptor fluid, while no long-chain CPs (LCCPs) (C>17) were able to penetrate the HSE during the 36-h assay period. The experimentally obtained permeability coefficient (K_p) values for CPs were significantly (p < 0.01) negatively correlated with their log octanol-water partition coefficient (log K_ow). Furthermore, 24 participants were recruited to assess direct human dermal exposure to CPs in China with the total CPs collected onto hand wipes and forehead wipes being 96,600 and 30,400 ng/person, respectively. The proportion of total SCCPs and MCCPs intake via dermal penetration (skin area investigated in this study) accounting for 2.0% of the total intake of CPs. Considering the total skin surface of human body is around 20 times of the area studied, the total intake of CPs through dermal penetration could be a significant exposure pathway.