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Updated: Oct 21, 2025

Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion
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Impedance-based Real-time Measurement of Cancer Cell Migration and Invasion

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CG7379 and ING1 suppress cancer cell invasion by maintaining cell-cell junction integrity

Alexandra D Rusu1,2, Zoe E Cornhill1, Brenda Canales Coutiño1,3

  • 1School of Life Sciences, University of Nottingham, Nottingham NG7 2UH, UK.

Open Biology
|September 8, 2021
PubMed

Insights

The fly gene CG7379, similar to human ING1, suppresses cancer cell invasion. Loss of this gene disrupts cell-cell junctions, promoting tumor spread.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Cancer Research

Background:

  • Tumor metastasis is a major cause of cancer mortality.
  • The ING1 gene is a known tumor suppressor involved in DNA damage response and cell cycle control.
  • The precise role of ING1 in cancer cell invasion and metastasis remains unclear.

Purpose of the Study:

  • To investigate the role of CG7379, the fly orthologue of human ING1, as a potential suppressor of cancer cell invasion.
  • To elucidate the molecular mechanisms by which ING1 influences cell-cell junction integrity and tumor metastasis.

Main Methods:

  • Utilized *Drosophila* *in vivo* models to study CG7379 function.
  • Performed *in vitro* experiments with ING1 knockdown in mammalian cells.
  • Analyzed cell-cell junction architecture and component localization.
  • Conducted transcriptome analysis to identify affected genes.

Main Results:

  • Reduced CG7379 expression in *Drosophila* promoted tumor invasion and disrupted adherens and septate junctions.
  • ING1 knockdown in mammalian cells enhanced invasion and disrupted E-cadherin localization at cell-cell junctions.
  • Loss of ING1 altered the expression of genes regulating cell junctions and the cytoskeleton.

Conclusions:

  • CG7379/ING1 acts as a potent suppressor of cancer cell invasion.
  • ING1 is crucial for maintaining cell-cell junction integrity.
  • ING1 regulates invasion through modulation of junctional and cytoskeletal components.
Keywords:
Drosophila melanogasterING1cancercell–cell junctionsinvasiontumour suppressor

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