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Related Experiment Video

Updated: Oct 21, 2025

A Phenotyping Regimen for Genetically Modified Mice Used to Study Genes Implicated in Human Diseases of Aging
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Mouse Ptchd3 is a non-essential gene.

Shaimar R González Morales1, Chengjing Liu1, Heath Blankenship1

  • 1Department of Biology, Marshall University, Huntington WV25755, USA.

Gene
|September 8, 2021
PubMed
Summary

The patched domain containing 3 (Ptchd3) gene is not essential for mouse development or sperm production. Knockout mice lacking Ptchd3 showed normal growth, fertility, and spermatogenesis, indicating no significant role in these processes.

Keywords:
Hedgehog signalingKnockoutMale germ cellsPtchd3Spermatogenesis

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Area of Science:

  • Reproductive biology
  • Developmental biology
  • Molecular genetics

Background:

  • The patched domain containing 3 (Ptchd3) gene is specifically expressed in male germ cells.
  • Ptchd3 protein is localized on the surface of sperm in mice, rats, and humans.
  • The presence of a conserved patched domain suggests a potential role as a membrane receptor for hedgehog ligands.

Purpose of the Study:

  • To investigate the function of Ptchd3 in mouse development and spermatogenesis using a knockout model.
  • To determine if Ptchd3 plays a role in the hedgehog signaling pathway.

Main Methods:

  • Generation and analysis of a Ptchd3 knockout mouse model.
  • Assessment of mouse development, viability, fertility, and sperm production.
  • Evaluation of hedgehog signaling gene expression in the testes of knockout mice.

Main Results:

  • Ptchd3 knockout mice exhibited normal development and viability.
  • Male knockout mice showed no alterations in fertility or sperm production.
  • Expression levels of key hedgehog signaling genes remained unaffected in the testes of mutant mice.

Conclusions:

  • Ptchd3 is not essential for normal mouse development.
  • Ptchd3 does not appear to play a critical role in spermatogenesis or male fertility.
  • The function of Ptchd3 in relation to hedgehog signaling in male germ cells requires further investigation.