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Related Concept Videos

Colloidal precipitates01:09

Colloidal precipitates

1.3K
The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
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Coagulation01:06

Coagulation

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Colloidal solids are solid particles suspended in solution. They are usually negatively charged, attracting a compact primary layer of positively charged ions, which attract more counterions to form an electrical double layer. Electrostatic repulsion between the charged double layers prevents the particles from colliding, stabilizing the colloids. These solids are often undesirable because they can contain toxins that are difficult to remove. Coagulation is a technique that helps aggregate and...
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Related Experiment Video

Updated: Oct 21, 2025

High-throughput Confocal Imaging of Quantum Dot-Conjugated SARS-CoV-2 Spike Trimers to Track Binding and Endocytosis in HEK293T Cells
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Colloidal aggregators in biochemical SARS-CoV-2 repurposing screens.

Henry R Oâ Donnell, Tia A Tummino, Conner Bardine

    Biorxiv : the Preprint Server for Biology
    |September 8, 2021
    PubMed
    Summary

    Many SARS-CoV-2 drug repurposing efforts may be compromised by colloidal aggregation artifacts. This study reveals that numerous drugs exhibit aggregation at concentrations relevant to biochemical assays, potentially leading to false positives in drug discovery.

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    Area of Science:

    • Drug discovery and development
    • Biochemistry and biophysics
    • Infectious disease research

    Background:

    • Drug repurposing is a key strategy for combating the SARS-CoV-2 pandemic.
    • Biochemical assays are widely used to screen drugs against viral and human targets.
    • Colloidal aggregation is a common artifact in early drug discovery that can lead to false positives.

    Approach:

    • Investigated the impact of colloidal aggregation in drug repurposing screens for SARS-CoV-2.
    • Tested 56 drugs for aggregation using dynamic light scattering and enzyme counter screening.
    • Evaluated 15 additional drugs with physical properties resembling known aggregators from a common repurposing library.

    Key Points:

    • Seventeen of 56 tested drugs formed colloids at concentrations near their reported IC50 values.
    • Six of 15 additional drugs aggregated at micromolar concentrations.
    • Colloidal aggregation can cause artifactual drug activity in biochemical assays at relevant concentrations.

    Conclusions:

    • A significant number of drugs repurposed for SARS-CoV-2 may yield false positive results due to aggregation.
    • Understanding and detecting colloidal aggregation is crucial for accurate drug screening.
    • Focusing on genuine drug leads will accelerate the development of effective COVID-19 treatments.