Protein-Assisted Osmium Nanoclusters with Intrinsic Peroxidase-like Activity and Extrinsic Antifouling Behavior

Extensive studies have laid the groundwork for understanding peroxidase-like nanozymes. However, improvements are still required before their practical applications. On one hand, it is significant to explore highly reactive nanozymes. On the other hand, it is necessary to avoid fouling formed on the surface of nanozymes, which will affect their activity and the results of H₂O₂ sensors or H₂O₂-related applications. Herein, a strategy is reported to design osmium nanoclusters (Os NCs) with the existence of bovine serum albumin (BSA) through biomineralization. BSA-Os NCs were found to possess intrinsic peroxidase-like activity with a high specific activity (6120 U/g). Studies also found that the catalytic activity of BSA-Os NCs was better than those of reported protein-assisted metal nanozymes (e.g., BSA-Pt NPs and BSA-Au NCs). More significantly, BSA has been confirmed as a protective shell to give Os NCs extrinsic antifouling property in some typical ions (e.g., Hg²⁺, Ag⁺, Pb²⁺, I^-, Cr⁶⁺, Cu²⁺, Ce³⁺, S^2-, etc.), saline (0-2 M), or protein (0-100 mg/mL) conditions. Under optimal conditions, a colorimetric sensor was established to realize a linear range of H₂O₂ from 1.25 to 200 μM with a low detection limit of 300 nM. On this basis, remarkable features enable a BSA-Os NCs-based colorimetric sensor to detect H₂O₂ from complex systems with clear color gradients. Together, this work highlights the advantages of protein-assisted Os nanozymes and provides a paragon for peroxidase-like nanozymes in H₂O₂-related applications.