Characterization of the corynebacteriophage CG33

Bacteriophage CG33 was isolated from a strain of Corynebacterium glutamicum that had become contaminated during an industrial fermentation. CG33 was assigned to Bradley's group B since it had a polyhedral head 40 nm wide and a short non-contractile and striated tail 78 nm long. Adsorption to its host, C. glutamicum ATCC 13287, was enhanced in the presence of Ca2+. The latent period was 18 min at 34 degrees C; the burst size was 16 p.f.u. ml-1. CG33 also formed plaques on C. lilium ATCC 15990 but at a low frequency. Its genome consisted of a linear double stranded DNA molecule of 13.4 kb with cohesive ends. A restriction map of the genome was obtained by using various endonucleases.