Related Experiment Video
Updated: Oct 21, 2025

Minimally Invasive Murine Laryngoscopy for Close-Up Imaging of Laryngeal Motion During Breathing and Swallowing
Published on: December 1, 2023
An in vitro assessment of light intensity provided during direct laryngeal visualization by videolaryngoscopes with
Madeleine Harlow1, George Kovacs2, Paul Brousseau3
1Dalhousie University, Halifax, NS, Canada.
Background:
Adequate illumination of the larynx is needed during laryngoscopy to facilitate tracheal intubation. The International Organization for Standardization (ISO) has established a minimum light intensity for direct laryngoscopy (DL) of over 500 lux for at least ten minutes, but no such standard exists for Macintosh geometry videolaryngoscope (Mac-VL) blades, which allow for both direct or indirect (videoscopic) viewing of the larynx. Using in situ bench and in vitro testing in a human cadaver, we determined illumination and luminance values delivered by various Mac-VLs and compared these with published minimum lighting benchmarks as well as a reference direct laryngoscope.
Methods:
We tested six Mac-VLs (i-view™, McGRATH™ MAC, GlideScope® Spectrum™ [single-use] DVM S4, GlideScope® Titanium [reusable] Mac T4, C-MAC® S [single-use] Macintosh #4, C-MAC® [reusable] Macintosh #4) together with one direct laryngoscope (Heine LED). Each laryngoscope was assessed with three measurements, as follows: part 1: illuminance (lux) was measured in situ using a purpose-designed benchtop light intensity measurement apparatus; part 2: luminance (light reflected back to the eye) was measured (in candela m-2 [cd·m-2]) during videolaryngoscopy (VL) and DL in a human cadaver using a spot meter pointed at the interarytenoid notch; part 3: illuminance (lux) was measured during VL and DL in a human cadaver using a light meter surgically implanted just proximal to the vocal cords.
Results:
Illuminance and luminance varied significantly among the Mac-VLs. Mean (standard devitation) illuminance among the six tested Mac-VLs ranged from 117 (11) to 2,626 (42) lux in the measurement apparatus and from 228 (11) to 2,900 (374) lux by the surgically implanted light meter in the cadaver. All values were less than the reference Heine direct laryngoscope and some fell below the published ISO standard of 500 lux for DL. Luminance testing by spot meter had a similarly wide range, varying from 3.78 (0.60) to 49.1 (10.4) cd·m-2, with some Mac-VLs delivering less luminance than the reference Heine direct laryngoscope.
Conclusions:
Our results indicate that illuminance and luminance provided by Mac-VLs used for direct laryngeal viewing varies substantially between devices, with some falling below standards previously suggested as the minimum required for DL. While this may have no implications for the quality of image visible on a device's video monitor, the clinician should be aware that when Mac-VLs are used for direct viewing of the larynx, lighting may not be optimal. This might adversely affect ease or success of tracheal intubation.
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