Real-time effects of Cd(II) on the cellular membrane permeability

Cell membrane permeability is one of the main indicators of cytotoxicity and related to many critical biological pathways. Here, we determined the Cd²⁺-induced membrane permeability of human MCF-7 cells using ferrocene methanol molecular probes based on scanning electrochemical microscopy (SECM). The cell height and topography were examined with an impermeable Ru(NH₃)₆Cl₃ probe. The membrane permeability exhibited no significant changes when the Cd²⁺ incubation time was less than 2 h and its concentration was less than 40 μM. The permeability increased when the Cd²⁺ concentration was greater than 60 μM, or when the incubation time was longer than 3 h. From the combined 3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and cytoskeleton imaging experiments, it was found that the changes occurred because the cells exhibited a defensive mode and their membranes contracted when treated with a low concentration of Cd²⁺ for a short time. However, the cell membranes were irreversibly damaged when the cytoskeleton structures were destroyed, and the cell activities decreased at high concentrations over long periods. Interestingly, through the comparison with an x-scan study, it was found that DPV technology shows a higher performance in the detection of changes in the membrane permeability. Using a combination of cytoskeleton fluorescence imaging and cell-viability tests, the effect of the cadmium metal on the cell membrane permeability can be explored deeper and more comprehensively. This study provides a new idea for exploring the changes in the cell membrane permeability and may be helpful for rapid evaluation of cytotoxicity.