Prussian blue immunochromatography with portable smartphone-based detection device for zearalenone in cereals

In this study, we developed a prussian blue nanoparticles (PBNPs) immunochromatographic assay (ICA) integrated with smartphone-based detection device for ZEN in cereals. PBNPs, as probe labels, were synthesized with properties of controllable structure, environment friendliness, and high affinities to antibody (Ab). PBNPs-ICA quantitative analysis was performed with a hand-held smartphone-based device coupled with a user-friendly and self-programmed detection App. This integrated strategy demonstrated high sensitivity for ZEN with a cut-off value of 10 μg/kg, a detection limit of 0.12 μg/kg, a quantitation limit of 0.27 μg/kg, and recovery rates of 92.0%-105.0% and 88.0%-98.0% for maize and wheat, respectively. The results of 20 naturally contaminated cereal samples showed good correlation (R²＞0.99) between LC-MS/MS and developed system. Moreover, the stability experiment revealed that PBNPs-ICA maintained high stability and bioactivity against competitive antigen (Ag). The proposed strategy exhibited great potential for the rapid monitoring of mycotoxins or other small molecule hazards.