Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Positive Regulator Molecules

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

Inhibition of Cdk Activity

Inhibition of Cdk Activity

The orderly progression of the cell cycle depends on the activation of Cdk protein by binding to its cyclin partner. However, the cell cycle must be restricted when undergoing abnormal changes. Most cancers correlate to the deregulated cell cycle, and since Cdks are a central component of the cell cycle, Cdk inhibitors are extensively studied to develop anticancer agents. For instance, cyclin D associates with several Cdks, such as Cdk 4/6, to form an active complex. The cyclin D-Cdk4/6 complex...

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

Anaphase Promoting Complex

Anaphase Promoting Complex

The stepwise destruction of specific proteins is necessary for the progression and completion of the cell cycle. Such proteins are ubiquitinated by ubiquitin ligases and then subsequently destroyed by the proteasome. The SCF (Skp1/Cullin/F-box) and the anaphase-promoting complex (APC) are two important ubiquitin ligases involved in cell cycle progression. While SCF is active throughout the cell cycle, APC gets activated during metaphase to anaphase transition. Cdc20 or Cdh1 binds to APC and...

MAPK Signaling Cascades

MAPK Signaling Cascades

Mitogen-activated protein kinase, or MAPK pathway, activates three sequential kinases to regulate cellular responses such as proliferation, differentiation, survival, and apoptosis. The canonical MAPK pathway starts with a mitogen or growth factor binding to an RTK. The activated RTKs stimulate Ras, which recruits Raf or MAP3 Kinase (MAPKKK), the first kinase of the MAPK signaling cascade. Raf further phosphorylates and activates MEK or MAP2 Kinases (MAPKK), which in turn phosphorylates MAP...

S-Cdk Initiates DNA Replication

S-Cdk Initiates DNA Replication

The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Uncovering cancer dependencies in peptide-interacting protein pockets.

bioRxiv : the preprint server for biology·2026

Same author

High-throughput investigation of cyclin docking interactions reveals the complexity of motif binding determinants.

Nature communications·2025

Same author

Phytate Content in Cereals Impacted by Cropping System and Harvest Year.

Foods (Basel, Switzerland)·2025

Same author

Ultrasound effect on a biorefinery lignin-cellulose mixture.

Ultrasonics sonochemistry·2024

Same author

Translational control of MPS1 links protein synthesis with the initiation of cell division and spindle pole body duplication in Saccharomyces cerevisiae.

Genetics·2024

Same author

CDK signaling via nonconventional CDK phosphorylation sites.

Molecular biology of the cell·2023

Search research articles

Related Experiment Video

Updated: Oct 20, 2025

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Docking to a Basic Helix Promotes Specific Phosphorylation by G1-Cdk1.

Ilona Faustova¹, Kaidi Möll¹, Ervin Valk¹

¹Institute of Technology, Faculty of Science and Technology, University of Tartu, 50411 Tartu, Estonia.

International Journal of Molecular Sciences

|September 10, 2021

Summary

Researchers discovered a new G1 cyclin-specific docking motif (K/R motif) in budding yeast. This motif is crucial for G1-cyclin-dependent kinase (CDK) targets, explaining essential cell survival functions.

Keywords:

SLiM cyclin specificity cyclin-dependent kinase kinase specificity phosphorylation

More Related Videos

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

Related Experiment Videos

Last Updated: Oct 20, 2025

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Combining Mitotic Cell Synchronization and High Resolution Confocal Microscopy to Study the Role of Multifunctional Cell Cycle Proteins During Mitosis

Published on: December 5, 2017

Oligopeptide Competition Assay for Phosphorylation Site Determination

Oligopeptide Competition Assay for Phosphorylation Site Determination

Published on: May 18, 2017

Area of Science:

Cell Biology
Molecular Biology
Biochemistry

Background:

Cyclin-dependent kinases (CDKs) coordinate the cell cycle through general activity and cyclin-specific substrate docking.
G1-cyclins in *S. cerevisiae* have essential roles in bud polarization and growth, but the underlying docking mechanism remains unknown.

Purpose of the Study:

To identify novel docking mechanisms employed by G1-CDKs in *S. cerevisiae*.
To elucidate the molecular basis for the essential function of G1-cyclins in budding yeast survival.

Main Methods:

Bioinformatic analysis to identify conserved motifs in G1-CDK targets.
Experimental validation of the identified motif's function in relevant cellular processes.
Biochemical assays to investigate the regulation of the motif by kinases.

Main Results:

Discovery of a G1 cyclin-specific lysine-arginine-rich helical docking motif (K/R motif) in *S. cerevisiae* G1-CDK targets.
The K/R motif is involved in mating, transcription, bud morphogenesis, and spindle pole body functions.
The docking efficiency of the K/R motif is regulated by basophilic kinases, such as protein kinase A.

Conclusions:

The K/R motif represents a novel mechanism for cyclin specificity in CDK function.
This finding expands the understanding of how G1-CDKs regulate essential cellular processes in budding yeast.
The K/R motif may explain the recently identified essential role of G1-cyclins in *S. cerevisiae* survival.