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Related Experiment Video

Updated: Oct 20, 2025

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

Published on: August 16, 2014

Complement Factor B Mediates Ocular Angiogenesis through Regulating the VEGF Signaling Pathway.

Hannah Murray¹, Beiying Qiu^2,3, Sze Yuan Ho^2,3,4

¹Institute of Molecular and Cell Biology, Agency for Science Technology and Research (A*STAR), Proteos, 61 Biopolis Dr., Singapore 138673, Singapore.

International Journal of Molecular Sciences

|September 10, 2021

Summary

Complement factor B (CFB) promotes pathological ocular neovascularization by mediating vascular endothelial growth factor (VEGF) signaling. This study clarifies CFB

Keywords:

VEGF angiogenesis complement factor B eye

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Last Updated: Oct 20, 2025

The Corneal Micropocket Assay: A Model of Angiogenesis in the Mouse Eye

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Published on: August 16, 2014

An Ex Vivo Choroid Sprouting Assay of Ocular Microvascular Angiogenesis

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Published on: August 6, 2020

In Vitro Model of Coronary Angiogenesis

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Area of Science:

Immunology
Ophthalmology
Angiogenesis Research

Background:

Complement factor B (CFB) is a key component of the complement alternative pathway (AP).
CFB's role in pathological ocular neovascularization is debated, necessitating further investigation.
Ocular neovascularization contributes to several blinding eye diseases.

Purpose of the Study:

To investigate the direct impact of Complement factor B (CFB) on ocular neovascularization.
To elucidate the mechanisms underlying CFB's role in pathological angiogenesis.

Main Methods:

Utilized mouse models of laser-induced choroidal neovascularization (CNV) and oxygen-induced retinopathy (OIR).
Conducted in vitro and ex vivo functionality assays.
Analyzed Complement factor B (CFB) expression and its effect on VEGF signaling.

Main Results:

Demonstrated increased CFB expression during pathological angiogenesis in mouse models.
Showcased a promoting effect of CFB on angiogenesis through in vitro and ex vivo assays.
Identified CFB's pro-angiogenic mechanism via mediation of the vascular endothelial growth factor (VEGF) signaling pathway.

Conclusions:

Complement factor B (CFB) plays a significant role in driving ocular angiogenesis.
CFB exerts its pro-angiogenic effects in a VEGF-dependent manner.
Findings provide a basis for future research into CFB-mediated ocular angiogenesis.