Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Real Time RT-PCR02:57

Real Time RT-PCR

62.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
62.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RNAi revised--target mRNA-dependent enhancement of gene silencing.

Nucleic acids research·2015
Same author

Technical improvements in the computational target search for antisense oligonucleotides.

Oligonucleotides·2005
See all related articles

Related Experiment Video

Updated: Oct 20, 2025

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.1K

Sampling, Logistics, and Analytics of Urine for RT-qPCR-based Diagnostics.

Rosel Kretschmer-Kazemi Far1, Kirsten Frank1, Georg Sczakiel1

  • 1Institut für Molekulare Medizin, Universität zu Lübeck and UKSH, Campus Lübeck, Ratzeburger Allee 160, 23538 Lübeck, Germany.

Cancers
|September 10, 2021
PubMed
Summary

This study presents a robust protocol for collecting and analyzing RNA biomarkers in urine for bladder cancer detection. The method ensures RNA stability for over 10 days, enabling reliable liquid biopsy analysis.

Keywords:
RNA isolationRNA stabilityRT-qPCRprimer designtumor marker

More Related Videos

Cell-Free DNA Integrity Analysis in Urine Samples
07:58

Cell-Free DNA Integrity Analysis in Urine Samples

Published on: January 5, 2017

14.0K
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
11:11

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Published on: February 11, 2022

4.7K

Related Experiment Videos

Last Updated: Oct 20, 2025

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging
04:33

Author Spotlight: Engineering Molecular Tools for Disease Detection and Imaging

Published on: December 8, 2023

1.1K
Cell-Free DNA Integrity Analysis in Urine Samples
07:58

Cell-Free DNA Integrity Analysis in Urine Samples

Published on: January 5, 2017

14.0K
Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
11:11

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots

Published on: February 11, 2022

4.7K

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Liquid biopsy offers a minimally invasive approach for cancer diagnosis using body fluids like urine.
  • RNA biomarkers in urine can reflect gene expression status in tumors, aiding in cancer monitoring.
  • Current methods for urine sample analysis require standardized protocols for reliable biomarker detection.

Purpose of the Study:

  • To develop and validate a comprehensive protocol for the robust acquisition and analysis of RNA-based tumor markers in urine.
  • To ensure the stability and integrity of RNA in urine samples during collection, transport, and storage.
  • To establish a reliable method for quantitative detection of RNA biomarkers for bladder cancer diagnosis.

Main Methods:

  • Detailed protocol for urine sample collection, stabilization, transportation, and storage.
  • Quantitative Polymerase Chain Reaction (qPCR)-based technology for RNA marker analysis.
  • Specific primer design and amplicon evaluation for accurate gene assignment.

Main Results:

  • The developed protocol ensures RNA stability in urine samples for over 10 days at ambient temperatures.
  • The method allows for specific assignment of PCR products to human genomic and transcriptomic data.
  • Demonstrated robustness for quantitative detection of RNA-based tumor markers in bladder cancer patients.

Conclusions:

  • A validated technical option for robust urine sample acquisition and RNA-based tumor marker detection in bladder cancer is presented.
  • The protocol is adaptable for analyzing any RNA-based tumor marker in urine from cancer patients.
  • This standardized approach enhances the utility of urine liquid biopsy for cancer diagnostics.