Naringin Exerts Therapeutic Effects on Mice Colitis: A Study Based on Transcriptomics Combined With Functional...

Area of Science:

• Gastroenterology
• Pharmacology
• Molecular Biology

Background:

• Ulcerative colitis (UC) is a chronic inflammatory bowel disease (IBD) with limited treatment options.
• Naringin shows protective effects in UC animal models, but its precise mechanisms are not fully understood.

Purpose of the Study:

• To elucidate the functional and signaling mechanisms of naringin's therapeutic effects in colitis.
• To identify key molecular targets of naringin in inflammatory bowel disease.

Main Methods:

• Established dextran sodium sulfate (DSS) and 2,4,6-trinitrobenzene sulfonic acid (TNBS) induced colitis mouse models.
• Utilized transcriptomics, in vivo, and in vitro functional assays to investigate naringin's effects.
• Examined the role of peroxisome proliferator-activated receptor gamma (PPAR-γ) and NF-κB signaling pathways.

Main Results:

• Naringin significantly alleviated colitis symptoms, including weight loss and disease activity index.
• Naringin reduced inflammation, oxidative stress, and epithelial cell apoptosis in colitis models.
• Transcriptomics revealed naringin impacts pathways including neuroactive ligand-receptor interaction, calcium signaling, and PPAR signaling.
• PPAR-γ inhibition blocked naringin's therapeutic effects; naringin suppressed LPS-induced NF-κB activation via PPAR-γ.

Conclusions:

• Naringin demonstrates significant therapeutic potential for ulcerative colitis.
• Peroxisome proliferator-activated receptor gamma (PPAR-γ) is identified as a key molecular target mediating naringin's anti-colitis effects.
• This study provides novel mechanistic insights into naringin's efficacy for IBD treatment.