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Updated: Oct 20, 2025

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PTEN downregulation induces apoptosis and cell cycle arrest in uterine cervical cancer cells

Jin Woo Shin1, Se-Hee Kim2, Jin Young Yoon2

  • 1Department of Obstetrics and Gynecology, Gil Medical Center, College of Medicine, Gachon University, Incheon 21565, Republic of Korea.

Experimental and Therapeutic Medicine
|September 10, 2021
PubMed

Insights

Silencing the PTEN gene in cervical cancer cells induces apoptosis and cell cycle arrest, decreasing proliferation. This suggests PTEN inhibition could be a promising cervical cancer treatment strategy.

Area of Science:

  • Oncology
  • Molecular Biology

Background:

  • Tumor suppressors PTEN and p53 are frequently downregulated in human cancers, correlating with poor prognosis.
  • PTEN downregulation is implicated in inducing growth arrest in kidney and cancer cells.

Purpose of the Study:

  • To investigate the role of PTEN in cervical cancer cell proliferation and survival.
  • To explore PTEN's impact on apoptosis and cell cycle progression in cervical cancer.

Main Methods:

  • PTEN silencing was performed in cervical cancer cells.
  • Cellular apoptosis, cell cycle phase distribution, and protein/gene expression levels were analyzed.
  • The influence of p53 silencing on PTEN knockdown effects was assessed.

Main Results:

  • PTEN silencing promoted apoptosis and cell-cycle arrest, reducing S1 phase cells.
  • Levels of p53, p27, p21, phospho-ERK, and cleaved caspase-3 increased, while cyclin A2 and cyclin D1 decreased.
  • PTEN knockdown significantly reduced cervical cancer cell viability, independently of p53.

Conclusions:

  • PTEN silencing induces apoptosis and reduces proliferation in cervical cancer cells.
  • PTEN inhibition presents a potential therapeutic strategy for cervical cancer treatment.
Keywords:
PTENapoptosiscervical cancerp53

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