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Updated: Oct 20, 2025
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Dry Root Rot Disease Assays in Chickpea: a Detailed Methodology
Published on: January 17, 2021
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A sick plot-based protocol for dry root rot disease assessment in field-grown chickpea plants
Vadivelmurugan Irulappan1, Komal Vitthalrao Mali1, Basavanagouda S Patil2
1National Institute of Plant Genome Research, Aruna Asaf Ali Marg New Delhi 110067 India.
Applications in Plant Sciences
|September 10, 2021
Summary
A new protocol establishes chickpea dry root rot (DRR) sick plots for effective disease screening. This method aids in assessing plant responses to Macrophomina phaseolina infection under field conditions.
Area of Science:
- Plant Pathology
- Agronomy
- Crop Science
Background:
- Dry root rot (DRR) in chickpea, caused by Macrophomina phaseolina, lacks a comprehensive field-based screening protocol.
- Effective disease assessment requires reliable methods for imposing infection under natural conditions.
Purpose of the Study:
- To describe a protocol for establishing a chickpea sick plot for DRR.
- To enable large-scale disease assessment during the natural growing season.
Main Methods:
- Developed a chickpea plot with over 30% DRR incidence.
- Enriched inoculum by cultivating susceptible genotypes and incorporating infected plant material.
- Subjected chickpea plants to M. phaseolina infection in sick plots under varying soil moisture levels and natural field conditions.
Main Results:
- Successfully established sick plots for DRR screening.
- Demonstrated the protocol's ability to impose M. phaseolina infection under natural field conditions.
- Facilitated assessment of chickpea responses to DRR at multiple biological levels.
Conclusions:
- The developed protocol offers a robust method for imposing M. phaseolina infection in chickpea.
- This approach enables comprehensive investigation of plant responses to DRR.
- The protocol is adaptable for screening other soil-borne diseases in various crops.
