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Updated: Oct 20, 2025

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Serum and Plasma Copy Number Detection Using Real-time PCR
Published on: December 15, 2017
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Circulating Cell-Free DNA-Based Detection of Tumor Suppressor Gene Copy Number Loss and Its Clinical Implication in
Xiaoxi Dong1, Tiantian Zheng1, Minhua Zhang2
1Predicine Inc., Hayward, CA, United States.
Frontiers in Oncology
|September 10, 2021
Abstract:
Current liquid biopsy assays lack sufficient sensitivity to detect copy number loss, which limits the interrogation of critical tumor suppressor gene deletions during cancer progression and treatment. Here we describe a liquid biopsy assay with improved sensitivity for detection of copy number loss in blood samples with low levels of circulating tumor DNA, and demonstrate its utility by profiling PTEN, RB1, and TP53 genetic loss in metastatic prostate cancer patients.

