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Serum circulating miRNA-342-3p as a potential diagnostic biomarker in parathyroid carcinomas: A pilot study
Julia Krupinova1, Natalya Mokrysheva1, Vasiliy Petrov1
1Endocrinology Research Center, Moscow, Russia.
Objective:
To compare the serum miRNA expression profiles between patients with benign and malignant parathyroid tumours.
Background:
Despite recent advances in molecular biology, a histological tissue biopsy is still the method of choice used to diagnose most cancers. The preoperative cytology is not an applicable method for diagnosis of parathyroid cancer (PC); therefore, huge interest exists in terms of finding alternative methodologies to seek specific cancer biomarkers.
Design:
A retrospective cross-sectional study.
Patients And Methods:
Serum samples of patients with PC (n = 13) and parathyroid adenoma (PA) (n = 11), age (p = .999) and sex (p = .999) were matched and examined via the simultaneous comparative expression analysis of 754 microRNAs (miRNAs). The «TaqMan OpenArray Human MicroRNA Panel» (Applied Biosystems) was used to conduct real-time PCRs using the «QuantStudio 12К Flex» station (Life Technologies).
Results:
According to the results of a pilot study, significant changes in expression levels between the PC group and the PA group (control) (p < .05) were observed for 17 miRNAs. Among them, the downregulation of miRNA-342-3p met the Benjamini-Hochberg adjustment criteria for multiple comparisons (p = .02).
Conclusions:
Serum miRNA-342-3p could be a promising biomarker for PC to improve diagnosis and prognosis.
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