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Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model

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Published on: April 6, 2021

Pseudomonas putida KT2440 endures temporary oxygen limitations.

Philipp Demling¹, Andreas Ankenbauer², Bianca Klein³

¹Institute of Applied Microbiology (iAMB), Aachen Biology and Biotechnology (ABBt), RWTH Aachen University, Aachen, Germany.

Biotechnology and Bioengineering

|September 10, 2021

Summary

Pseudomonas putida, a key biocatalyst, can tolerate temporary oxygen limitations common in large bioreactors. This robustness confirms its suitability for industrial bioprocesses despite initial growth deceleration.

Keywords:

Pseudomonas putida metabolic engineering plug flow reactor rhamnolipids scale-down temporary oxygen limitation

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Area of Science:

Microbial Biotechnology
Bioprocess Engineering
Synthetic Biology

Background:

Pseudomonas putida is a prominent whole-cell biocatalyst for industrial applications.
Obligate aerobic nature of P. putida raises concerns for large-scale cultivation in bioreactors with low dissolved oxygen tension.

Purpose of the Study:

To assess the impact of temporary oxygen limitations on P. putida KT2440 growth and rhamnolipid production.
To evaluate the robustness of P. putida KT2440 under conditions simulating industrial bioreactors.

Main Methods:

Scale-down approach simulating temporary oxygen limitations in bioreactors.
Cultivation of P. putida KT2440 under varying oxygen tensions and nutrient conditions.
Quantification of intracellular nucleotides (ATP, ADP, AMP) and proteome analysis.

Main Results:

Growth and rhamnolipid production were decelerated but final biomass and titers remained similar under oxygen limitation.
Robust growth was observed across different cultivation systems, media, and laboratories.
Intracellular ATP decreased with oxygen starvation but was restored upon re-oxygenation; proteome showed minimal changes.

Conclusions:

P. putida KT2440 demonstrates resilience to repeated oxygen limitations encountered in large-scale bioreactors.
The bacterium's ability to cope with oxygen fluctuations affirms its suitability as an industrial whole-cell biocatalyst.