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Published on: October 23, 2011
RAPD PCR detects co-colonisation of multiple group B streptococcus genotypes: A practical molecular technique for
Ka-Ning To1, Oliver Powell2, Dorota Jamrozy3
1Department of Infectious Disease, Imperial College London, Norfolk Place, London W2 1PG, UK; Institute of Infection and Immunity, St George's University of London, Cranmer Terrace, London SW17 0RE, UK.
None:
Group B Streptococcus (GBS) is a leading cause of neonatal meningitis, pneumonia, and sepsis. The biggest contributing factor of neonatal infections is due to vertical transmission from maternal colonisation of GBS in the genitourinary tract. Multiple serotype colonisation is often not investigated in epidemiological studies, but it is an important consideration for serotype-based vaccine development and implementation to ensure less abundant serotypes are not under-represented. In this study, we show that RAPD PCR is a quick tool useful in screening the presence of genetically different strains using multiple colony picks from a single patient swab. We observed a maximum of five different GBS strains colonising a single patient at a specific time.
