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In vitro effect of trifluoperazine on human lymphocytes
A Suryanarayana1, P Rita, P P Reddy
1Institute of Genetics, Osmania University, Begumpet, Hyderabad, Aandra Pradesh, India.
The Japanese Journal of Experimental Medicine
|October 1, 1987
Summary
Trifluoperazine, a tranquilizer, was found to damage human chromosomes, causing breaks and gaps in lymphocytes. This phenothiazine drug primarily affects the late S and G2 phases of the cell cycle.
Area of Science:
- Cytogenetics
- Pharmacology
- Cell Biology
Background:
- Trifluoperazine is a phenothiazine tranquilizer.
- Phenothiazines are known for various pharmacological effects.
- Understanding drug-induced genotoxicity is crucial for safety.
Purpose of the Study:
- To investigate the chromosome damaging potential of trifluoperazine.
- To determine the dose- and time-dependent effects of trifluoperazine on human lymphocytes.
- To identify the cell cycle phases affected by trifluoperazine.
Main Methods:
- Human lymphocyte cultures were treated with trifluoperazine at concentrations of 10, 15, and 20 micrograms/culture.
- Cultures were incubated for 24, 48, and 72 hours.
- Chromosomal aberrations, including breaks and gaps, were analyzed.
Main Results:
- A significant increase in chromatid breaks and gaps was observed.
- The incidence of chromosomal damage was higher after 48 and 72 hours of exposure.
- Trifluoperazine demonstrated a genotoxic effect on human lymphocytes.
Conclusions:
- Trifluoperazine induces chromosomal damage in human lymphocytes.
- The drug's genotoxicity is evident at higher concentrations and longer exposure times.
- Trifluoperazine appears to primarily target the late S and G2 phases of the cell cycle.