Glutathione modulated fluorescence quenching of sulfur quantum dots by Cu₂O nanoparticles for sensitive assay

Sulfur quantum dots (S-dots) show great potential for applications in various field, due to their favorable biocompatibility, high stability, and antibacterial properties. However, the use of S-dots in chemical sensing is limited by the lack of functional groups on the surface. In this work, a fluorescence glutathione (GSH) assay is developed based on the GSH modulated quenching effect of Cu₂O nanoparticles (NP) on S-dots. The fluorescence of S-dots is effectively quenched after forming complex with Cu₂O NP through a static quenching effect (SQE). Introducing of GSH can trigger the decomposition of Cu₂O NP into GSH-Cu(I) complex, which leads to the weaken of SQE and the partial recover of the fluorescence. The intensity of recovered fluorescence shows a positive correlation with the concentration of GSH in the concentration range of 20 to 500 μM. The fluorescence GSH assay shows excellent selectivity and robustness towards various interferences and high concentration salt, which endow the successful detection of GSH in human blood sample. The presented results provide a new door for the design of fluorescence assays, which also provides a platform for the applications in nanomedicine and environmental science.