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Updated: Oct 20, 2025

Amplification of Escherichia coli in a Continuous-Flow-PCR Microfluidic Chip and Its Detection with a Capillary Electrophoresis System
Published on: November 21, 2023
Overview of in-capillary enzymatic reactions using capillary electrophoresis
Jef Rozenski1, Adissu Alemayehu Asfaw2,3, Ann Van Schepdael2
1Department ofPharmaceutical and Pharmacological Sciences, Medicinal Chemistry, Rega Institute, KU Leuven-University of Leuven, Leuven, Belgium.
Abstract:
This review summarizes the research that has recently been performed on in-capillary enzymatic reactions integrated with capillary electrophoresis. The manuscript is subdivided in homogeneous and heterogeneous approaches. The main homogeneous techniques are Electrophoretically Mediated Microanalysis, At-inlet and Transverse Diffusion of Laminar Flow Profiles. The main heterogeneous ones are Immobilized MicroEnzyme Reactors with enzymes grafted on either non-magnetic or magnetic particles. The overview covers the period from 2018 to early 2021. The applications range from drug discovery over natural products to food, beverage and pesticide analysis.
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