Related Experiment Video

Updated: Oct 20, 2025

Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells
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Isolation and Flow Cytometric Analysis of Glioma-infiltrating Peripheral Blood Mononuclear Cells

Published on: November 28, 2015

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FOXN3 inhibits cell proliferation and invasion via modulating the AKT/MDM2/p53 axis in human glioma

Chaojia Wang1, Hanjun Tu2, Ling Yang3

  • 1Department of Neurosurgery, Taihe Affiliated Hospital of Hubei University of Medicine, Shiyan 442000, China.

Aging
|September 13, 2021
PubMed

Insights

Forkhead box N3 (FOXN3) is downregulated in glioma, suppressing tumor growth and invasion. Restoring FOXN3 inhibits proliferation and motility by inactivating the AKT/MDM2/p53 pathway, offering a potential therapeutic target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Glioma is a primary brain tumor with limited therapeutic options.
  • The role of forkhead box N3 (FOXN3) in glioma pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the biological role of FOXN3 in human glioma.
  • To elucidate the molecular mechanisms underlying FOXN3's function in glioma.

Main Methods:

  • Quantitative PCR and Western blotting for FOXN3 expression analysis.
  • Cell proliferation, apoptosis, migration, and invasion assays (CCK8, colony formation, flow cytometry, scratch wound, Transwell).
  • In vivo tumor xenograft models and analysis of the AKT/MDM2/p53 signaling pathway.

Main Results:

  • FOXN3 expression is significantly downregulated in glioma tissues.
  • Lower FOXN3 levels correlate with shorter patient survival.
  • FOXN3 overexpression suppresses glioma cell proliferation, survival, and motility, while FOXN3 knockdown promotes these.
  • FOXN3 inactivates the AKT/MDM2/p53 signaling pathway, inhibiting tumor growth in vitro and in vivo.

Conclusions:

  • FOXN3 acts as a tumor suppressor in glioma.
  • The FOXN3-AKT/MDM2/p53 axis represents a potential therapeutic target for glioma treatment.
Keywords:
AKT/MDM2/p53 pathwayFOXN3cell migrationcell proliferationglioma

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