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Updated: Oct 20, 2025

Diagnosis of Hirschsprung's Disease by Immunostaining Rectal Suction Biopsies for Calretinin, S100 Protein and Protein Gene Product 9.5
Published on: April 26, 2019
Sanne J Verkuijl1, Florian Friedmacher1, Patrick N Harter2
1Department of Pediatric Surgery, University Hospital Frankfurt, Goethe University Frankfurt, Frankfurt 60590, Germany.
Hirschsprung's disease (HD) involves missing nerves in the gut. Even after surgery, many patients experience ongoing bowel issues due to abnormalities in the remaining bowel nerves.
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