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Published on: March 15, 2013
Full-length transcriptome analysis of Spodoptera frugiperda larval brain reveals detoxification genes
Lei Yang1, Binglin Xing1, Fen Li1
1Hainan University, Haikou, Hainan, China.
Background:
Spodoptera frugiperda (J. E. Smith), commonly known as fall armyworm (FAW), is one of the most destructive agricultural pests in the world and has posed a great threat to crops. The improper use of insecticides has led to rapid development of resistance. However, the genetic data available for uncovering the insecticide resistance mechanisms are scarce.
Methods:
In this study, we used PacBio single-molecule real-time (SMRT) sequencing aimed at revealing the full-length transcriptome profiling of the FAW larval brain to obtain detoxification genes.
Results:
A total of 18,642 high-quality transcripts were obtained with an average length of 2,371 bp, and 11,230 of which were successfully annotated in six public databases. Among these, 5,692 alternative splicing events were identified.
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