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Related Experiment Video

Updated: Oct 20, 2025

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Detection and Monitoring of Tumor Associated Circulating DNA in Patient Biofluids

Published on: June 8, 2019

Tumor Biomarker In-Solution Quantification, Standard Production, and Multiplex Detection.

Nicole C Japp¹, Joshua J Souchek¹, Aaron R Sasson^1,2

¹Sanguine Diagnostics and Therapeutics, Inc., Omaha, Nebraska, USA.

Journal of Immunology Research

|September 13, 2021

Summary

Discovering novel cancer biomarkers and enhancing detection sensitivity are crucial for improving patient outcomes. This review covers advancements in serum/plasma protein biomarker analysis, including paramagnetic bead assays and multiplexing for sensitive detection.

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Last Updated: Oct 20, 2025

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Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

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Published on: April 11, 2016

Area of Science:

Biomarker Discovery and Development
Clinical Diagnostics and Monitoring
Assay Technology and Automation

Background:

Sensitive and specific biomarkers are essential for cancer diagnosis and monitoring, yet remain elusive for certain cancers.
Patient liquid biopsies (serum/plasma) offer accessible noninvasive sources for protein biomarker analysis.
Existing enzyme-linked immunosorbent assay (ELISA) approaches face limitations in dynamic range and automation.

Purpose of the Study:

To review the evolution of biomarker standards and assay technologies for serum/plasma protein analysis.
To highlight advancements in chemiluminescent and fluorescent detection methods for biomarker quantification.
To discuss the application of multiplexing strategies for sensitive detection of novel biomarkers in limited sample volumes.

Main Methods:

Review of historical biomarker standards and development of paramagnetic particle assay principles.
Examination of chemiluminescent and fluorescent detection technologies applied to biomarker assays.
Analysis of multiplexing techniques for simultaneous detection of multiple biomarkers.

Main Results:

Paramagnetic bead-based assays offer improved dynamic range and automation for clinical biomarker testing.
Chemiluminescent and fluorescent detection methods enhance sensitivity in biomarker quantification.
Multiplexing enables efficient analysis of multiple biomarkers from limited serum volumes, crucial for research settings.

Conclusions:

Advancements in assay technology, including paramagnetic beads and sensitive detection methods, are critical for improving cancer biomarker analysis.
Multiplexing strategies are vital for the sensitive detection of novel serum biomarkers, particularly in research and development.
Continued innovation in biomarker discovery and assay sensitivity is key to advancing cancer diagnostics and patient care.