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Related Experiment Video

Updated: Oct 20, 2025

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Published on: July 7, 2014

FDISCO+: a clearing method for robust fluorescence preservation of cleared samples.

Peng Wan^1,2, Yusha Li^1,2, Jingtan Zhu^1,2

¹Huazhong University of Science and Technology, Britton Chance Center for Biomedical Photonics, Wuhan National Laboratory for Optoelectronics, Wuhan, China.

|September 13, 2021

Summary

A new method, FDISCO+, modifies solvent-based optical clearing for room temperature storage. This technique preserves fluorescent signals effectively, simplifying long-term sample imaging and storage for researchers.

Keywords:

FDISCO+antioxidant fluorescence imaging fluorescence preservation optical clearing room temperature

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Last Updated: Oct 20, 2025

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO

Published on: July 7, 2014

Optical Clearing of Plant Tissues for Fluorescence Imaging

Optical Clearing of Plant Tissues for Fluorescence Imaging

Published on: January 5, 2022

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Optical Clearing of the Mouse Central Nervous System Using Passive CLARITY

Published on: June 30, 2016

Area of Science:

Neuroscience
Biotechnology
Microscopy

Background:

Solvent-based optical clearing methods like FDISCO excel at preserving fluorescent signals.
However, FDISCO requires strict low-temperature storage, limiting its utility for room-temperature, long-term, or repetitive imaging.

Purpose of the Study:

To develop a modified FDISCO method (FDISCO+) that enables room-temperature storage while maintaining fluorescence preservation.
To address the incompatibility between FDISCO's preservation requirements and standard laboratory imaging conditions.

Main Methods:

Screening of two alternative antioxidants to inhibit peroxide generation in the clearing agent at room temperature.
Modification of the original FDISCO protocol to adapt it for room-temperature conditions.

Main Results:

FDISCO+ demonstrates fluorescence preservation comparable to the original FDISCO protocol.
The modified method allows for extended sample storage at room temperature, facilitating easier imaging and preservation.

Conclusions:

FDISCO+ offers a practical solution for optical clearing, overcoming the temperature limitations of previous methods.
The relaxed operational requirements of FDISCO+ are anticipated to promote its widespread adoption in biological research.