Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

RNA Splicing01:32

RNA Splicing

57.8K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
57.8K
Alternative RNA Splicing02:18

Alternative RNA Splicing

22.0K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
22.0K
Chromatin Structure and RNA Splicing02:41

Chromatin Structure and RNA Splicing

2.9K
2.9K
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

7.5K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
7.5K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

5.7K
5.7K
Ribosome Profiling02:24

Ribosome Profiling

3.7K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Generation of ciBMECs: Endothelial Cells Acquire Blood-Brain Barrier Identity and Function Through Wnt Activation.

Arteriosclerosis, thrombosis, and vascular biology·2026
Same author

Rapid centromere turnover and the adaptive radiation of lemurs.

bioRxiv : the preprint server for biology·2026
Same author

Reciprocal repulsions enforce heterotypic dendrite segregation in an olfactory circuit.

bioRxiv : the preprint server for biology·2026
Same author

Engulfment by brain macrophages in a short-lived vertebrate.

bioRxiv : the preprint server for biology·2026
Same author

TranscriptFormer: A generative cell atlas across 1.5 billion years of evolution.

Science (New York, N.Y.)·2026
Same author

Integrating and mapping single-cell transcriptomics across the entire gene expression space.

Briefings in bioinformatics·2026
Same journal

The exquisite mechanics of a tsetse bite.

eLife·2026
Same journal

Distinct involvements of the subthalamic nucleus subpopulations in reward-biased decision-making in monkeys.

eLife·2026
Same journal

Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses during inflammation.

eLife·2026
Same journal

Restraint of melanoma progression by cells in the local skin environment.

eLife·2026
Same journal

Brawn before bite in endemic Asian eutherian mammals after the end-Cretaceous extinction.

eLife·2026
Same journal

Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.

eLife·2026
See all related articles

Related Experiment Video

Updated: Oct 20, 2025

Author Spotlight: Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining
08:51

Author Spotlight: Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining

Published on: April 21, 2023

3.7K

RNA splicing programs define tissue compartments and cell types at single-cell resolution.

Julia Eve Olivieri1,2,3, Roozbeh Dehghannasiri2,3, Peter L Wang3

  • 1Institute for Computational and Mathematical Engineering, Stanford University, Stanford, United States.

Elife
|September 13, 2021
PubMed
Summary
This summary is machine-generated.

Cell-type-specific splicing regulation was controversial. A new method, SpliZ, analyzed over 110,000 cells, revealing widespread cell-type-specific splicing across human tissues.

Keywords:
RNAcomputational biologygeneticsgenomicshumanmousemouse lemurscRNA-seqsplicingstatisticssystems biology

More Related Videos

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

19.2K
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.9K

Related Experiment Videos

Last Updated: Oct 20, 2025

Author Spotlight: Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining
08:51

Author Spotlight: Dissection of Adult Mouse Stria Vascularis for Single-Nucleus Sequencing or Immunostaining

Published on: April 21, 2023

3.7K
Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing
06:38

Isolation of Adult Spinal Cord Nuclei for Massively Parallel Single-nucleus RNA Sequencing

Published on: October 12, 2018

19.2K
Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models
09:58

Using RNA-sequencing to Detect Novel Splice Variants Related to Drug Resistance in In Vitro Cancer Models

Published on: December 9, 2016

13.9K

Area of Science:

  • Molecular Biology
  • Genomics
  • Bioinformatics

Background:

  • The regulation of RNA splicing at the single-cell level has been a complex and debated topic.
  • Existing data and analytical methods have limited the ability to precisely determine cell-type-specific splicing patterns.
  • Understanding cell-type-specific splicing is crucial for comprehending cellular function and diversity.

Purpose of the Study:

  • To develop and apply a novel statistical approach, SpliZ, for detecting cell-type-specific splicing.
  • To investigate the extent of splicing regulation at single-cell resolution across diverse human tissues.
  • To identify specific genes and cellular subpopulations exhibiting differential splicing patterns.

Main Methods:

  • Application of the SpliZ statistical method to analyze splicing patterns.
  • Utilized large-scale single-cell RNA sequencing data (>110,000 cells) from 12 human tissues (10X Chromium).
  • Validated findings using complementary techniques such as RNA Fluorescence In Situ Hybridization (FISH), single-cell PCR, and Smart-seq2.

Main Results:

  • 9.1% of genes with computable SpliZ scores demonstrated cell-type-specific splicing, including ubiquitously expressed genes like MYL6 and RPS24.
  • Identified 170 genes with regulated splicing during human spermatogenesis, with conserved examples in mouse and mouse lemur.
  • Discovered subpopulations with distinct splicing patterns, such as classical monocytes with ultraconserved exon splicing in SAT1, undetectable by gene expression alone.

Conclusions:

  • Splicing is extensively regulated in a cell-type-specific manner across multiple human organs.
  • The SpliZ approach enables the identification of cellular subpopulations based on splicing signatures.
  • This study provides a comprehensive resource for understanding cell-type-specific splicing regulation in humans.