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DrFLINC Contextualizes Super-resolution Activity Imaging
Wei Lin1, Gary C H Mo1, Sohum Mehta1
1Department of Pharmacology, University of California, San Diego, La Jolla, California 92093, United States.
Abstract:
Super-resolution activity imaging maps the biochemical architecture of living cells yet currently overlooks the locations of collaborating regulators/effectors. Building on the fluorescence fluctuation increase by contact (FLINC) principle, here we devise Dronpa-chromophore-removed FLINC (DrFLINC), where the nonfluorescent Dronpa can nevertheless enhance TagRFP-T fluorescence fluctuations. Exploiting DrFLINC, we develop a superior red label and a next-generation activity sensor for context-rich super-resolution biosensing.
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