In-Field Capable Loop-Mediated Isothermal Amplification Detection of Spodoptera frugiperda (Lepidoptera: Noctuidae)...

Area of Science:

• Agricultural Entomology
• Molecular Diagnostics
• Pest Management

Background:

• The fall armyworm (Spodoptera frugiperda) is a significant global pest, now present in Australia.
• Early larval identification of fall armyworm is challenging, hindering timely management.
• There is a need for rapid, field-deployable diagnostic tools for fall armyworm detection.

Purpose of the Study:

• To develop a sensitive, specific, and in-field capable loop-mediated isothermal amplification (LAMP) assay for detecting fall armyworm (Spodoptera frugiperda) larvae.
• To provide a tool for rapid and accurate in-field diagnosis to support management decisions.

Main Methods:

• Development of a loop-mediated isothermal amplification (LAMP) assay using a primer set targeting a conserved region of the S. frugiperda cytochrome oxidase subunit 1 (COX1) gene.
• Utilized a simple, rapid crude extraction method involving crushing larval material.
• Tested assay sensitivity down to 24 pg of S. frugiperda DNA and specificity against common Australian lepidopteran species.

Main Results:

• The developed LAMP assay detected S. frugiperda within 30 minutes using both total DNA and crude extracts.
• The crude extraction method is simple, field-friendly, and does not require specialized equipment.
• The assay demonstrated high sensitivity and specificity, with no cross-reactivity with other tested lepidopteran species.

Conclusions:

• The developed LAMP assay is a sensitive, specific, and rapid tool for in-field detection of fall armyworm (Spodoptera frugiperda) larvae.
• This assay can significantly aid in early and accurate identification, facilitating timely and effective pest management strategies in Australia.
• The field-deployable nature of the assay enhances its practical utility for agricultural stakeholders.