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Related Experiment Video

Updated: Oct 20, 2025

Forced Flowering in Mandarin Trees under Phytotron Conditions
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Florigen sequestration in cellular membranes modulates temperature-responsive flowering.

Hendry Susila1, Snježana Jurić1,2, Lu Liu3,4

  • 1Department of Life Sciences, Korea University, Seoul 02841, Korea.

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Plants use cellular membranes to control flowering time. The FLOWERING LOCUS T (FT) protein binds the phospholipid phosphatidylglycerol (PG) in membranes, regulating flowering responses to temperature.

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Area of Science:

  • Plant Biology
  • Molecular Biology
  • Plant Physiology

Background:

  • Flowering time in plants is crucial for reproductive success and is influenced by environmental cues like temperature.
  • The mobile florigen FLOWERING LOCUS T (FT) is a key regulator of the transition to flowering.
  • Understanding how FT activity is modulated by environmental factors is essential for crop improvement.

Purpose of the Study:

  • To investigate the molecular mechanism by which temperature affects FT activity and flowering time in *Arabidopsis thaliana*.
  • To determine the role of cellular membranes and specific phospholipids in FT regulation.
  • To identify potential targets for modulating plant temperature responses.

Main Methods:

  • Biochemical assays to study the interaction between FT and phospholipids.
  • Genetic analysis using *Arabidopsis thaliana* mutants, including those affecting phosphatidylglycerol (PG) biosynthesis.
  • Microscopy and biochemical methods to quantify soluble and membrane-bound FT levels under different temperature conditions.

Main Results:

  • The FT protein directly interacts with and binds to the negatively charged phospholipid phosphatidylglycerol (PG) at cellular membranes.
  • Disruption of PG biosynthesis in phloem companion cells results in temperature-insensitive early flowering.
  • Low temperatures promote FT sequestration into cellular membranes, reducing soluble FT levels and delaying flowering; a *PHOSPHATIDYLGLYCEROLPHOSPHATE SYNTHASE 1* mutant shows increased soluble FT at low temperatures.

Conclusions:

  • Cellular membranes, through binding of the phospholipid PG, act as a reservoir to sequester FT.
  • This membrane sequestration of FT is a critical mechanism by which plants modulate flowering time in response to ambient temperature.
  • The FT-PG interaction provides a novel target for understanding and potentially engineering plant thermoperiodism.