Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.5K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.5K
Role of Hematopoietic Growth Factors01:28

Role of Hematopoietic Growth Factors

1.9K
Hematopoietic growth factors are molecules that regulate the differentiation rate of hematopoietic stem cells (HSCs). Erythropoietin (EPO), primarily produced by the kidneys, plays a crucial role in erythrocyte production. When oxygen levels in the blood are low, EPO is released into the bloodstream, reaching the bone marrow, where it stimulates HSCs to differentiate and mature into erythrocytes, which are vital for oxygen transport.
Thrombopoietin (TPO), mainly released by the liver,...
1.9K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

5.4K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
5.4K
Hematopoiesis01:21

Hematopoiesis

7.1K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
7.1K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.4K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.4K
Lineage Commitment01:21

Lineage Commitment

3.5K
Commitment is the  process whereby stem cells:
3.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Multifaceted roles of PDS5B in RAD51-dependent homology-directed DNA repair and replication fork protection.

Nature communications·2026
Same author

Glycerol-mediated nose-to-brain codelivery of anti-IL-17 and anti-CD73 antibodies enhances immunotherapy for melanoma brain metastases.

Science advances·2026
Same author

Structural insight into how RAD51 paralog exchange regulates RAD51 filament formation.

Nature structural & molecular biology·2026
Same author

Resolution of R-loops and transcription-replication conflicts by SETX-BRCA1-BARD1 complex.

Nature structural & molecular biology·2026
Same author

Cryo-EM structures of UBA6 reveal mechanisms of E1-E2 specificity and dual FAT10/ubiquitin thioester transfer.

Nature communications·2026
Same author

Upregulated ZBP1 Is Associated with B-Cell Dysregulation in Systemic Lupus Erythematosus.

Biomedicines·2026

Related Experiment Video

Updated: Oct 20, 2025

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.6K

INTS11 regulates hematopoiesis by promoting PRC2 function.

Peng Zhang1, Pinpin Sui1, Shi Chen2

  • 1Department of Cell Systems & Anatomy, University of Texas Health Science Center at San Antonio, San Antonio, TX 78229, USA.

Science Advances
|September 13, 2021
PubMed
Summary
This summary is machine-generated.

Integrator complex subunit 11 (INTS11) is vital for hematopoietic stem and progenitor cell (HSPC) function. Loss of INTS11 impairs hematopoiesis by destabilizing the Polycomb repressive complex 2 (PRC2).

More Related Videos

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.6K
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

9.6K

Related Experiment Videos

Last Updated: Oct 20, 2025

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.6K
Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro
06:12

Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Published on: March 7, 2022

3.6K
Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells
10:25

Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

Published on: August 9, 2019

9.6K

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Hematology

Background:

  • Integrator complex subunit 11 (INTS11) is essential for RNA biogenesis.
  • The function of INTS11 in hematopoietic stem and progenitor cell (HSPC) biology remains uncharacterized.

Purpose of the Study:

  • To investigate the role of INTS11 in maintaining HSPC function and hematopoiesis.
  • To identify molecular mechanisms by which INTS11 regulates HSPCs.

Main Methods:

  • Hematopoietic-specific genetic deletion of Ints11 in mice.
  • Analysis of cell cycle, proliferation, and differentiation of HSPCs.
  • Co-immunoprecipitation assays to identify INTS11-interacting proteins.
  • Western blotting to assess protein levels and histone modifications (H3K27me3).

Main Results:

  • Genetic deletion of Ints11 in HSPCs caused cell cycle arrest and impaired fetal and adult HSPC function.
  • A novel INTS11-interacting complex, Polycomb repressive complex 2 (PRC2), was identified.
  • Loss of INTS11 led to PRC2 destabilization, reduced H3K27me3 levels, and derepression of PRC2 target genes.
  • Re-expression of INTS11 or PRC2 restored PRC2 levels, H3K27me3, and HSPC functions.

Conclusions:

  • INTS11 is indispensable for normal hematopoiesis and HSPC homeostasis.
  • The INTS11-PRC2 axis is a critical regulatory pathway for maintaining HSPC function.
  • INTS11 regulates HSPC function by stabilizing PRC2 and influencing its epigenetic targets.