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Determination of spermatozoa concentration in the rat cauda epididymidis
T L Lamano-Carvalho1, W G Kempinas
1Departamento de Estomatologia, Faculdade de Odontologia de Ribeirão Preto, Universidade de São Paulo, Brasil.
Abstract:
A method for estimating the concentration of spermatozoa in the rat cauda epididymidis is described. Treatment of a sperm suspension with 0.05% collagenase for 20-60 min or 0.025% trypsin for 1-2 min at 34-37 degrees C was found to result in consistently homogeneous sperm. Sperm concentration ranged from 152.5 to 230.0 x 10(7) spermatozoa/ml, with a mean of 187.7 +/- 5.6 (SEM) x 10(7) spermatozoa/ml.