Naphthalimide appended isoquinoline fluorescent probe for specific detection of Al³⁺ ions and its application in...

Herein, a novel Schiff base fluorescent probe NIQ based on naphthalimide and iso-quinoline units has been readily prepared for the selective detection of Al³⁺ ions. The obviously visible color changes and prominent fluorescence enhancement were observed upon the addition of Al³⁺ to NIQ, which could be attributed to the complexation of NIQ with Al³⁺ and thus leading to the inhibition of photo-induced electron transfer (PET) and the chelation-enhanced fluorescence (CHEF) progress. The limit of detection (LOD) was 52 nM that was far below the standard recommended by the WHO. Binding ratio (1:1) of NIQ with Al³⁺ ions was supported by Job's plot. The binding constant of NIQ for Al³⁺ were calculated to be 3.27 × 10⁵ M^-1 on the basis of benesi-Hildebrand plot. The plausible binding mechanism for NIQ towards Al³⁺ ions was evidenced by the density functional theory (DFT) and ¹H NMR titration experiment. Furthermore, this "turn-on" probe NIQ has been successfully applied as a biomarker for imaging the Al³⁺ ions in living cells.