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Updated: Oct 20, 2025

Large Scale Non-targeted Metabolomic Profiling of Serum by Ultra Performance Liquid Chromatography-Mass Spectrometry UPLC-MS

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Published on: March 14, 2013

Comparing ELISA and LC-MS-MS: A Simple, Targeted Postmortem Blood Screen.

Dina M Swanson^1,2, Julia M Pearson¹, Theresa Evans-Nguyen²

¹Hillsborough County Medical Examiner Department, 11025 N. 46th Street, Tampa, FL 33617, USA.

Journal of Analytical Toxicology

|September 14, 2021

Summary

This summary is machine-generated.

A new liquid chromatography-tandem mass spectrometry (LC-MS-MS) method offers accurate and cost-effective drug screening in postmortem blood. This validated forensic toxicology method outperforms traditional enzyme-linked immunosorbent assay (ELISA) testing.

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High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry

Published on: April 23, 2019

Area of Science:

Forensic Toxicology
Analytical Chemistry
Mass Spectrometry

Background:

Immunoassays are common in forensic toxicology for drug screening due to cost-effectiveness and sensitivity.
Increasing accessibility of mass spectrometry necessitates re-evaluation of screening methods.
A need exists for specific, accurate, and customizable screening methods in forensic labs.

Purpose of the Study:

To develop and validate a comprehensive screening method for 42 drugs in postmortem blood.
To compare the performance of a novel LC-MS-MS method against traditional ELISA.
To assess the method's suitability for forensic laboratories within budget constraints.

Main Methods:

Developed and validated a drug screening method for postmortem blood using liquid chromatography-tandem mass spectrometry (LC-MS-MS).
Employed minimal sample preparation involving only protein precipitation.
Validated the method according to Organization of Scientific Area Committees for Forensic Science guidelines, including LOD, interference, stability, and ion suppression studies.
Analyzed over 100 case samples comparing LC-MS-MS to ELISA.

Main Results:

The LC-MS-MS method demonstrated comparable or superior performance to ELISA in nearly all analyzed cases.
The method requires minimal sample preparation and uses only two internal standards, reducing costs.
Limit of detection, interference, stability, and ion suppression were thoroughly examined.

Conclusions:

The developed LC-MS-MS method is a reliable, accurate, and cost-effective alternative for drug screening in forensic toxicology.
This method offers improved specificity and the flexibility to add more drug targets.
It is ideal for forensic laboratories seeking enhanced screening capabilities while managing costs.