Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Isolation01:24

DNA Isolation

42.6K
DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
42.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ensemble Modeling of Shifts in the Suitable Distribution and Ecological Niche of the Alpine Tibetan Medicinal Herb <i>Corydalis hendersonii Hemsl.</i> Under Climate Change and Human Activity.

Ecology and evolution·2026
Same author

Accelerating Moss Identification Through the Development of Specific DNA Barcodes Based on the Whole Chloroplast Genome.

Molecular ecology resources·2025
Same author

Chloroplast Genome Evolution of Hamamelidaceae at Subfamily Level.

Ecology and evolution·2025
Same author

Comparative study on chloroplast genome of <i>Tamarix</i> species.

Ecology and evolution·2024
Same author

What determines plant species diversity along the Modern Silk Road in the east?

iMeta·2024
Same author

Synthesis and herbicidal activity of optically active α-(substituted phenoxyacetoxy) (substituted phenyl) methylphosphonates.

Pesticide biochemistry and physiology·2017
Same journal

High Gene Flow Despite Urbanization: Genetic Diversity and Structure of the Eastern Spotted Dove (<i>Spilopelia chinensis</i>) in Jiangsu Province, China and Implications for Releasing Confiscated Individuals.

Ecology and evolution·2026
Same journal

Evidence of Small Changes in Daytime Body Temperature in Active Black-Capped Chickadees in Response to Supplemental Food Availability.

Ecology and evolution·2026
Same journal

Seasonal Variation of Butterfly Diversity in Subtropical Urban Forests of Nepal.

Ecology and evolution·2026
Same journal

Influences of Carrion Decomposition on Soil Nutrient Leakage in a Boreal Forest.

Ecology and evolution·2026
Same journal

Salamander-<i>Batrachochytrium salamandrivorans</i> Interactions Through Dual Transcriptomics.

Ecology and evolution·2026
Same journal

Flower Feeding and Reproductive Timing in Spix's Night Monkeys (<i>Aotus vociferans</i>): Evidence From Arboreal Camera Traps.

Ecology and evolution·2026
See all related articles

Related Experiment Video

Updated: Oct 20, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

10.8K

Method for quick DNA barcode reference library construction.

Yanlei Liu1,2, Chao Xu1, Yuzhe Sun1,2

  • 1State Key Laboratory of Systematic and Evolutionary Botany Institute of Botany Chinese Academy of Sciences Beijing China.

Ecology and Evolution
|September 15, 2021
PubMed
Summary
This summary is machine-generated.

We present efficient methods for DNA barcoding to build comprehensive plant species identification databases. Our approach uses next-generation sequencing and a novel Cotu method to reduce costs and accelerate library construction.

Keywords:
CotuDNA barcodedata processing methodnext‐generation sequencing

More Related Videos

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.8K
Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.5K

Related Experiment Videos

Last Updated: Oct 20, 2025

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens
13:03

Robust DNA Isolation and High-throughput Sequencing Library Construction for Herbarium Specimens

Published on: March 8, 2018

10.8K
Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq
06:24

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

3.8K
Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.5K

Area of Science:

  • Botany
  • Genomics
  • Bioinformatics

Background:

  • DNA barcoding is crucial for plant species identification, but database construction faces challenges.
  • High species coverage in reference databases is essential for accurate plant identification.

Purpose of the Study:

  • To address experimental and data processing challenges in constructing plant DNA barcoding reference databases.
  • To evaluate next-generation sequencing platforms and develop efficient methods for DNA barcoding.

Main Methods:

  • Sequencing of six conventional DNA barcode fragments from 380 flowering plants using Illumina Hiseq 2500, Ion Torrent S5, and Sanger sequencing.
  • Comparison of sequencing performance metrics across platforms to determine suitability for DNA barcoding.
  • Development of the "Cotu" method for generating consensus sequences from next-generation sequencing reads.

Main Results:

  • Illumina Hiseq2500 PE250 runs are suitable for conventional DNA barcoding.
  • The "Cotu" method produces longer and more reliable consensus sequences compared to other methods.
  • High-throughput sequencing, PCR labeling, and the Cotu method significantly reduce cost and labor.

Conclusions:

  • Efficient DNA barcoding methods can overcome current limitations in database construction.
  • The developed approach facilitates the rapid creation of extensive DNA barcoding reference libraries.
  • A regional or global DNA barcoding reference library with high species coverage is achievable in the near future.