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Evaluation of Colorectal Cancer Risk and Prevalence by Stool DNA Integrity Detection

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A simple and sensitive method to detect vitamin D receptor expression in various disease models using stool samples.

Yong-Guo Zhang¹, Yinglin Xia¹, Jun Sun^1,2,3

¹Division of Gastroenterology and Hepatology, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA.

Genes & Diseases

|September 15, 2021

Summary

A new quantitative PCR (qPCR) method uses fecal samples to detect reduced intestinal Vitamin D receptor (VDR) expression. This sensitive technique offers a simple, non-invasive way to assess VDR status in various diseases.

Keywords:

Aging Biomarker Correlation Infection Inflammation Microbiome Salmonella Vitamin D deficiency

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Last Updated: Oct 20, 2025

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Published on: June 8, 2020

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Visualization of Estrogen Receptors in Colons of Mice with TNBS-Induced Crohn's Disease using Immunofluorescence

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Published on: March 12, 2020

Area of Science:

Molecular Biology
Gastroenterology
Immunology

Background:

The Vitamin D receptor (VDR) is crucial for immunity, barrier function, and gut microbiome regulation.
Reduced intestinal VDR is linked to diseases like inflammatory conditions and colon cancer.
Intestinal biopsies for VDR assessment are invasive and challenging.

Purpose of the Study:

To develop a simple and sensitive quantitative PCR (qPCR) method for detecting reduced intestinal VDR using fecal samples.
To validate the qPCR method in various experimental disease models.
To correlate fecal VDR qPCR data with intestinal VDR protein levels and serum vitamin D metabolites.

Main Methods:

Development and validation of a qPCR assay for VDR detection in fecal samples.
Application of the assay in experimental models of colitis, bacterial infection, and aging.
Correlation analysis between fecal VDR qPCR, colonic VDR protein, and serum 25(OH)D3 levels in mice with varying VDR genotypes (VDR+/+, VDR+/-, VDR-/-).

Main Results:

The developed qPCR method successfully detected reduced intestinal VDR expression in fecal samples.
The method demonstrated sensitivity and feasibility across different experimental models.
Fecal VDR qPCR results correlated with VDR protein levels and vitamin D status.

Conclusions:

A non-invasive qPCR method using fecal samples can effectively assess intestinal VDR status.
This molecular approach is sensitive, simple, and feasible for studying VDR in various disease contexts.
Fecal VDR detection serves as a potential biomarker for intestinal VDR changes.