Optimizing temperature for enhancing waste activated sludge decomposition in lysozyme and rhamnolipid pretreatment...

This study investigated the influence of temperature on the hydrolysis and decomposition of waste activated sludge (WAS) during the enhanced pretreatment system with lysozyme and rhamnolipid (Ly + RL). Results showed that temperature increasing from 15℃ to 55℃ could obviously enhance the release of soluble organic matters and WAS decomposition degree within the Ly + RL pretreatment system. Compared to the sum of sole Ly and sole RL pretreatment, Ly + RL combined pretreatment system at 45℃ presented best synergistic hydrolysis performance. The decomposition degree of bacteria and archaea reached 47.6% and 88.1%, respectively. Meanwhile, increasing temperature could recede the diversity of microbial community in the system. Gammaproteobacteria, with the relative abundance of 90.7%, occupied the absolute dominant position at 45℃. Furthermore, with the rise of temperature, more volatile fatty acids were harvested after anaerobic fermentation.