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Updated: Oct 20, 2025

Glutamine Flux Imaging Using Genetically Encoded Sensors
Published on: July 31, 2014
Fluorescent glutamine and asparagine as promising probes for chemical biology
Kiran Bajaj1, Karishma Pidiyara1, Shahid Khan2
1Department of Chemistry, Birla Institute of Technology and Science, Pilani 333031, Rajasthan, India. kbajaj26@gmail.com.
Abstract:
Fluorescent probes have become valuable tools in chemical biology, providing interesting inferences for unfolding the complexities of natural biochemical processes. In this study, we report the synthesis and characterization of fluorescent labelled glutamine (Gln) and asparagine (Asn) derivatives via traceless Staudinger ligation, which exhibited high fluorescence quantum yields, excellent photostabilities and emission of blue fluorescence in the visible region. The successful permeation of these fluorescent amino acids into cellular components proved their potential as fluorescent probes for chemical biology.
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