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Methods and cell-based strategies to produce antibody libraries: current state
Paula Matos de Brito1, Andreia Saruga1,2, Miguel Cardoso1
1Faculty of Pharmacy, iMed.ULisboa - Research Institute for Medicines, University of Lisbon, Av. Professor Gama Pinto, 1649-019, Lisbon, Portugal.
Applied Microbiology and Biotechnology
|September 15, 2021
Summary
Therapeutic antibodies are vital for fighting diseases. New platforms in higher eukaryotic cells mimic natural antibody diversification, improving discovery and engineering of these promising drugs.
Area of Science:
- Immunology
- Biotechnology
- Drug Discovery
Background:
- Antibodies are key elements of adaptive immunity with expanding therapeutic uses.
- Current antibody discovery methods include in vivo immunization, synthetic libraries, and surface display.
Purpose of the Study:
- To describe recent strategies for mirroring antibody repertoire in higher eukaryotic cells.
- To address limitations of existing antibody discovery and engineering platforms.
Main Methods:
- Development of novel platforms in higher eukaryotic cells.
- Replication of in vivo antibody diversification mechanisms (primary and secondary).
- Strategies to mirror naive and affinity-matured antibody repertoires.
Main Results:
- Innovative antibody discovery platforms have been established.
- These platforms aim to replicate natural antibody diversification processes.
- Engineered antibodies can be generated from hybridoma or display technologies.
Conclusions:
- Advanced platforms in higher eukaryotic cells offer improved antibody discovery and engineering.
- These methods enhance the development of therapeutic antibodies.
- Future therapeutic applications of antibodies are promising.
