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A simple method for the quantitative evaluation of phagocytosis and intracellular killing by peripheral blood
E F Oliveira1, A B Assis, E A Marques
1Departamento de Patologia, Faculdade de Ciências Médicas, Universidade Estadual do Rio de Janeiro, Brasil.
Abstract:
Several immunodeficiency disorders result from phagocytic or intracellular killing defects. Evaluation is frequently impaired by the complexity of available tests. A new and simple method is presented for the evaluation of the phagocytic and intracellular killing activity by peripheral blood leukocytes, using a small blood volume. Normal individuals show a phagocytic index of 61.4 +/- 16.9% (range 44.5 - 78.3%) after 2 h and 73.1 +/- 13.0% (range 60.1 - 86.1%) after 3 h incubation. For the same incubation periods, the intracellular killing indices were 84.6 +/- 11.0% (range 73.6 - 95.6%) and 88.3 +/- 11.6%) (76.7 -99.9%), respectively.