Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

17.6K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
17.6K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

10.4K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
10.4K
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.2K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Triple parabolic cone wheel: a driving structure for the rapid panoramic tracking imaging system.

Applied optics·2026
Same author

Hierarchical bayesian fusion of inspection and monitoring data for probabilistic bridge deterioration assessment.

Scientific reports·2026
Same author

Association of benzene exposure and atrial fibrillation in a national cohort: The modifying effect of genetic susceptibility.

Ecotoxicology and environmental safety·2025
Same author

Genome-wide association study reveals <i>CBX7</i> as a novel susceptibility gene associated with primary spontaneous pneumothorax in the Chinese Han population.

The European respiratory journal·2025
Same author

Dl-3-n-Butylphthalide enhances the survival of rat bone marrow stem cells via a reactive oxygen species mediated Erk1/2 signaling pathway.

Brain research·2025
Same author

Cross-Cultural Adaption and Psychometric Evaluation of Chinese Version of the Caregiver Contribution to Self-Care of Heart Failure Index.

Research in nursing & health·2025

Related Experiment Video

Updated: Oct 20, 2025

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

13.7K

Single-shot imaging through scattering media under strong ambient light interference.

Wei Li, Teli Xi, Shunfu He

    Optics Letters
    |September 15, 2021
    PubMed
    Summary

    Speckle correlation imaging (SCI) now works in noisy conditions. A new method refines speckle data, enabling high-quality single-shot imaging even with significant background noise.

    More Related Videos

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.4K
    Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
    10:55

    Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

    Published on: November 20, 2018

    17.6K

    Related Experiment Videos

    Last Updated: Oct 20, 2025

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
    11:57

    Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

    Published on: May 20, 2013

    13.7K
    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
    10:16

    Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects

    Published on: February 8, 2014

    12.4K
    Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy
    10:55

    Label-Free Imaging of Single Proteins Secreted from Living Cells via iSCAT Microscopy

    Published on: November 20, 2018

    17.6K

    Area of Science:

    • Optics and Photonics
    • Biomedical Imaging
    • Image Reconstruction

    Background:

    • Speckle correlation imaging (SCI) offers versatile, single-shot data acquisition.
    • Traditional SCI requires darkroom conditions due to sensitivity to background noise.
    • Low signal-to-background ratios (SBR) severely degrade image quality and data interpretation.

    Purpose of the Study:

    • To develop a robust SCI method adaptable to low signal-to-background ratio (SBR) environments.
    • To enhance speckle data processing for improved imaging performance.
    • To validate the method in both laboratory and outdoor settings.

    Main Methods:

    • Speckle data refinement in the autocorrelation domain.
    • Development of a novel single-shot imaging technique.
    • Experimental validation under varying SBR conditions.

    Main Results:

    • Achieved high-performance single-shot imaging in low SBR conditions.
    • Demonstrated successful object estimation with SBR as low as -23 dB.
    • Validated the method's efficacy in both controlled laboratory and real-world outdoor experiments.

    Conclusions:

    • The refined speckle autocorrelation method significantly enhances SCI robustness.
    • This technique overcomes the limitations of darkroom requirements for SCI.
    • Enables reliable imaging in challenging, noisy environments, expanding SCI applications.