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Updated: Oct 20, 2025

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces
Published on: March 19, 2010
EndoBind detects endogenous protein-protein interactions in real time
Anke Bill1, Sheryll Espinola2, Daniel Guthy3
1Novartis Institute for Biochemical Research, Oncology, Cambridge, MA, USA. anke.bill@novartis.com.
Abstract:
We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CRAF-KRAS-G12V interaction, we demonstrate that our method allows for the detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of endogenous protein-protein interactions in real time.
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