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Related Concept Videos

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Berberine Acts on C/EBPβ/lncRNA Gas5/miR-18a-5p Loop to Decrease the Mitochondrial ROS Generation in HK-2 Cells.

Jiang Xu1, Linqing Liu2, Lin Gan3

  • 1Department of Endocrinology, The First Affiliated Hospital of University of Science and Technology of China (USTC), Division of Life Sciences and Medicine, University of Science and Technology of China, Hefei, China.

Frontiers in Endocrinology
|September 16, 2021
PubMed
Summary
This summary is machine-generated.

Berberine (BBR) treats diabetic nephropathy by activating a C/EBPβ/Gas5/miR-18a-5p feedback loop, reducing mitochondrial ROS and renal injury.

Keywords:
C/EBPβGas5berberinediabetic nephropathyreactive oxygen species

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Area of Science:

  • Molecular Biology
  • Cell Biology
  • Renal Physiology

Background:

  • Diabetic nephropathy (DN) is a complication of diabetes with unclear molecular mechanisms.
  • Berberine (BBR) shows therapeutic potential for DN, but its precise molecular targets require elucidation.

Purpose of the Study:

  • To investigate the molecular mechanism of Berberine (BBR) in treating diabetic nephropathy (DN).
  • To explore the role of the C/EBPβ/Gas5/miR-18a-5p axis in BBR's therapeutic effects.

Main Methods:

  • Establishment of a DN model in rats and HK-2 cells.
  • Quantitative PCR, ChIP-qPCR, and luciferase reporter assays to analyze gene and protein interactions.
  • Assessment of mitochondrial reactive oxygen species (ROS) generation and apoptosis.

Main Results:

  • Berberine (BBR) reduced mitochondrial ROS and activated C/EBPβ expression in high glucose (HG) conditions.
  • C/EBPβ promoted Gas5 expression, which acted as a competing endogenous RNA (ceRNA) to inhibit miR-18a-5p.
  • BBR activated the C/EBPβ/Gas5/miR-18a-5p positive feedback loop, regulating mitochondrial function and alleviating renal injury in vivo.

Conclusions:

  • Berberine (BBR) regulates mitochondrial ROS generation in diabetic nephropathy (DN) by activating the C/EBPβ/Gas5/miR-18a-5p positive feedback loop.
  • This pathway represents a novel therapeutic target for DN treatment.