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Updated: Oct 20, 2025

Characterization of Functionally Associated miRNAs in Glioblastoma and their Engineering into Artificial Clusters for Gene Therapy
Published on: October 4, 2019
miR-190a-3p Promotes Proliferation and Migration in Glioma Cells via YOD1
Lili Zhou1, Lingzhi Li2, Yan Chen3
1Department of Neurology, The Affiliated Huai'an Hospital of Xuzhou Medical University, Huai'an, 223002 Jiangsu, China.
Introduction:
To investigate the function of miR-190a-3p on the proliferation and migration of glioma.
Methods:
Twenty glioma samples and 6 normal brain tissue samples were collected. Normal human glial cell line HEB and glioma cell lines were used for the experiments. We then used TargetScan to predict the target genes of miR-190a-3p. Dual-luciferase reporter assay was also used to validate.
Results:
Combined with dual-luciferase reporter experiment, we finally verified that YOD1 was the aim, and it was low-expressed in glioma. Besides, a series of mechanism experiments then proved that miR-190a-3p negatively regulates YOD1 expression.
Conclusions:
Our research was the first to demonstrate the promoting function of miR-190a-3p in the proliferation and migration of glioma and provided new views for the treatment of glioma. miR-190a-3p was expected to be a new target for molecular therapy of glioma.
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