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Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
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MedProDB: A database of Mediator proteins
Rohan Bhardwaj1,2, Jitendra Kumar Thakur2,3, Shailesh Kumar1
1Bioinformatics Lab, National Institute of Plant Genome Research (NIPGR), Aruna Asaf Ali Marg, New Delhi 110067, India.
Computational and Structural Biotechnology Journal
|September 16, 2021
Summary
Researchers developed MedProDB, the first database of Mediator proteins, aiding in the study of eukaryotic gene transcription regulation. This resource provides extensive data and analysis tools for Mediator complex research.
Area of Science:
- Molecular Biology
- Bioinformatics
- Genomics
Background:
- The multi-subunit Mediator complex is crucial for eukaryotic gene transcription.
- Bioinformatics advancements enable in-silico characterization of Mediator subunits across kingdoms.
Purpose of the Study:
- To develop the first comprehensive database of Mediator proteins, named MedProDB.
- To provide a centralized resource for researchers studying transcriptional regulation.
Main Methods:
- Compiled 33,971 Mediator protein sequences from metazoans, plants, and fungi.
- Integrated core and additional features including sequence properties, functions, interactions, IDRs, PTMs, and MoRFs.
- Incorporated analysis tools like BLAST, Smith-Waterman Align, IUPred, and MoRF-Chibi_Light.
Main Results:
- MedProDB contains 33,971 Mediator protein entries, with detailed information and analysis capabilities.
- The database includes sequences from metazoans (12,531), plants (11,545), and fungi (9,895).
- Provides diverse data on protein characteristics, functions, and potential modifications.
Conclusions:
- MedProDB serves as a valuable resource for structural and functional studies of the Mediator complex.
- Facilitates research into the fundamental process of transcription in eukaryotes.
- The database is freely accessible for scientific community use.
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