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The Role of Biofilms in Chronic Otitis Media-Active Squamosal Disease: An Evaluative Study
Gautam Bir Singh1, Sonali Malhotra1, Subhash Chandra Yadav2
1Department of Otorhinolaryngology - Head & Neck Surgery, Lady Hardinge Medical College & Associated Hospitals.
Objectives:
To study the presence of biofilms in patients of chronic otitis media (COM)-active squamosal disease and to evaluate the microflora and clinical impact of biofilms.
Methods:
A total of 35 patients suffering from COM - active squamosal disease was studied. Cholesteatoma sample was collected at the time of mastoid surgery and the same was used to image for biofilms by scanning electron microscope. The said sample was also analyzed microbiologically.
Results:
Biofilms were present in 25 (72%) patients including 6 cases of fungal biofilms (24%). Biofilms were detected irrespective of the micro-organism growth. No statistically significant relationship was observed between biofilms and discharging ear and complications of COM. Though univariate analysis of our data found some correlation between duration of disease, the extent of cholesteatoma and ossicular necrosis but multivariate analysis revealed no such associations, thereby implying a doubtful association between these factors and biofilm.
Conclusions:
Biofilms are an integral part of cholesteatoma ear disease and sometimes harbors fungal isolates.
